Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the detection of extended spectrum β-lactamases and carbapenemases among Gram-negative bacilli
MALDI-TOF-MS hydrolysis assay for β-lactamase detection
Keywords:
Carbapenemases, extended spectrum β-lactamases, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, resistance to β-lactam antibioticsAbstract
In the last few years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has become the new gold standard method for the identification of microorganisms. Nevertheless, other useful applications of mass spectrometry have recently been demonstrated including the detection of resistance mechanisms. The MALDI-TOF-MS hydrolysis assay allows rapid detection of β-lactamase activity with results available within a few hours. Rapid detection of β-lactamases is important since β-lactam antibiotics remains as the mainstay of the treatment of several community-acquired and nosocomial infections, and a steady increase has been observed of resistant microorganisms associated with the production of extended spectrum β-lactamases (ESBLs) and carbapenemases. Several studies have reported the high sensitivity and specificity of the MALDI-TOF-MS antibiotic hydrolysis assay for the detection of ESBLs - or carbapenemase-producing Gram-negative bacilli. In addition, this technique can be performed directly from positive blood culture, significantly shortening the time of resistance detection. However, the standardization of assay conditions as well as automatic analysis of the spectra obtained is required. The aim of this review was to summarize the currently available data regarding the accuracy of the detection of β-lactamase activity using the MALDI-TOF-MS antibiotic hydrolysis assay.
References
Welker M. Proteomics for routine identification of microorganisms. Proteomics 2011;11:3143-53.
Clark AE, Kaleta EJ, Arora A, Wolk DM. Matrix-assisted laser desorption ionization-time of flight mass spectrometry: A fundamental shift in the routine practice of clinical microbiology. Clin Microbiol Rev 2013;26:547-603.
Vila J, Juiz P, Salas C, Almela M, de la Fuente CG, Zboromyrska Y, et al. Identification of clinically relevant Corynebacterium spp. Arcanobacterium haemolyticum, and Rhodococcus equi by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2012;50:1745-7.
Tudó G, Monté MR, Vergara A, López A, Hurtado JC, Ferrer-Navarro M, et al. Implementation of MALDI-TOF MS technology for the identification of clinical isolates of Mycobacterium spp. in mycobacterial diagnosis. Eur J Clin Microbiol Infect Dis 2015;34:1527-32.
Pinto A, Halliday C, Zahra M, van Hal S, Olma T, Maszewska K, et al. Matrix-assisted laser desorption ionization-time of flight mass spectrometry identification of yeasts is contingent on robust reference spectra. PLoS One 2011;6:e25712.
Calderaro A, Arcangeletti MC, Rodighiero I, Buttrini M, Gorrini C, Motta F, et al. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry applied to virus identification. Sci Rep 2014;4:6803.
Calderaro A, Piergianni M, Buttrini M, Montecchini S, Piccolo G, Gorrini C, et al. MALDI-TOF mass spectrometry for the detection and differentiation of Entamoeba histolytica and Entamoeba dispar. PLoS One 2015;10:e0122448.
La Scola B, Raoult D. Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry. PLoS One 2009;4:e8041.
Juiz PM, Almela M, Melcion C, Campo I, Esteban C, Pitart C, et al. A comparative study of two different methods of sample preparation for positive blood cultures for the rapid identification of bacteria using MALDI-TOF MS. Eur J Clin Microbiol Infect Dis 2012;31:1353-8.
Ferreira L, Sanchez-Juanes F, Gonzalez-Avila M, Cembrero-Fucinos D, Herrero-Hernandez A, Gonzalez-Buitrago JM, et al. Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2010;48:2110-5.
Novais Â, Sousa C, de Dios Caballero J, Fernandez-Olmos A, Lopes J, Ramos H, et al. MALDI-TOF mass spectrometry as a tool for the discrimination of high-risk Escherichia coli clones from phylogenetic groups B2 (ST131) and D (ST69, ST405, ST393). Eur J Clin Microbiol Infect Dis 2014;33:1391-9.
Hrabák J, Chudácková E, Walková R. Matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry for detection of antibiotic resistance mechanisms: From research to routine diagnosis. Clin Microbiol Rev 2013;26:103-14.
Gagnaire J, Dauwalder O, Boisset S, Khau D, Freydière AM, Ader F, et al. Detection of Staphylococcus aureus delta-toxin production by whole-cell MALDI-TOF mass spectrometry. PLoS One 2012;7:e40660.
Kumar A, Roberts D, Wood KE, Light B, Parrillo JE, Sharma S, et al. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 2006;34:1589-96.
Kumar A, Ellis P, Arabi Y, Roberts D, Light B, Parrillo JE, et al. Initiation of inappropriate antimicrobial therapy results in a fivefold reduction of survival in human septic shock. Chest 2009;136: 1237-48.
Wybo I, De Bel A, Soetens O, Echahidi F, Vandoorslaer K, Van Cauwenbergh M, et al. Differentiation of cfiA-negative and cfiA-positive Bacteroides fragilis isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2011;49:1961-4.
Camara JE, Hays FA. Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Bioanal Chem 2007;389:1633-8.
Hrabák J, Walková R, Studentová V, Chudácková E, Bergerová T. Carbapenemase activity detection by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2011;49:3222-7.
Hrabák J, Studentová V, Walková R, Zemlicková H, Jakubu V, Chudácková E, et al. Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 carbapenemases by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2012;50:2441-3.
Burckhardt I, Zimmermann S. Using matrix-assisted laser desorption ionization-time of flight mass spectrometry to detect carbapenem resistance within 1 to 2.5 hours. J Clin Microbiol 2011;49:3321-4.
Sparbier K, Schubert S, Weller U, Boogen C, Kostrzewa M. Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based functional assay for rapid detection of resistance against beta-lactam antibiotics. J Clin Microbiol 2012;50:927-37.
Kempf M, Bakour S, Flaudrops C, Berrazeg M, Brunel JM, Drissi M, et al. Rapid detection of carbapenem resistance in Acinetobacter baumannii using matrix-assisted laser desorption ionization-time of flight mass spectrometry. PLoS One 2012;7:e31676.
Studentova V, Papagiannitsis CC, Izdebski R, Pfeifer Y, Chudackova E, Bergerova T, et al. Detection of OXA-48-type carbapenemase-producing Enterobacteriaceae in diagnostic laboratories can be enhanced by addition of bicarbonates to cultivation media or reaction buffers. Folia Microbiol (Praha) 2015;60:119-29.
Carvalhaes CG, Cayô R, Visconde MF, Barone T, Frigatto EA, Okamoto D, et al. Detection of carbapenemase activity directly from blood culture vials using MALDI-TOF MS: A quick answer for the right decision. J Antimicrob Chemother 2014;69:2132-6.
Hoyos-Mallecot Y, Riazzo C, Miranda-Casas C, Rojo-Martín MD, Gutiérrez-Fernández J, Navarro-Marí JM. Rapid detection and identification of strains carrying carbapenemases directly from positive blood cultures using MALDI-TOF MS. J Microbiol Methods 2014;105:98-101.
Lasserre C, De Saint Martin L, Cuzon G, Bogaerts P, Lamar E, Glupczynski Y, et al. Efficient detection of carbapenemase activity in Enterobacteriaceae by matrix-assisted laser desorption ionization-time of flight mass spectrometry in less than 30 minutes. J Clin Microbiol 2015;53:2163-71.
Chong PM, McCorrister SJ, Unger MS, Boyd DA, Mulvey MR, Westmacott GR. MALDI-TOF MS detection of carbapenemase activity in clinical isolates of Enterobacteriaceae spp. Pseudomonas aeruginosa, and Acinetobacter baumannii compared against the Carba-NP assay. J Microbiol Methods 2015;111:21-3.
Knox J, Jadhav S, Sevior D, Agyekum A, Whipp M, Waring L, et al. Phenotypic detection of carbapenemase-producing Enterobacteriaceae by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and the Carba NP test. J Clin Microbiol 2014;52:4075-7.
Jung JS, Popp C, Sparbier K, Lange C, Kostrzewa M, Schubert S. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid detection of ß-lactam resistance in Enterobacteriaceae derived from blood cultures. J Clin Microbiol 2014;52:924-30.
Oviaño M, Fernández B, Fernández A, Barba MJ, Mouriño C, Bou G. Rapid detection of Enterobacteriaceae producing extended spectrum beta-lactamases directly from positive blood cultures by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Clin Microbiol Infect 2014;20:1146-57.
Li B, Guo T, Qu F, Li B, Wang H, Sun Z, et al. Matrix-assisted laser desorption ionization: Time of flight mass spectrometry-identified models for detection of ESBL-producing bacterial strains. Med Sci Monit Basic Res 2014;20:176-83.
Downloads
Published
How to Cite
Issue
Section
Downloads
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
