Gut Microbiota and Integrative Wellness
https://www.hksmp.com/journals/gmiw
<p>Gut Microbiota and Integrative Wellness (GMIW) is an internationally peer-reviewed, open-access journal published in English language. GMIW focuses on basic and translational research, and issues of patient care, across Traditional and Western Medicine in the fields of gastroenterology.</p>en-USeditorialoffice@gmiw-journal.com (Wison Zhang)stanleyzhang@sppub.org (Stanley Zhang)Wed, 03 Apr 2024 15:13:54 +0800OJS 3.3.0.7http://blogs.law.harvard.edu/tech/rss60The Qizhi Weitong Granules Alleviate Functional Dyspepsia-like Gastric Hypersensitivity in Rats by Inhibiting Inflammatory Cytokines and Strengthening Duodenal Mucosal Barrier
https://www.hksmp.com/journals/gmiw/article/view/532
<p><strong>Background</strong>: Qizhi Weitong (QZWT) granules are a compound preparation of Chinese medicine, which are approved by the<br />China Food and Drug Administration for the treatment of functional dyspepsia (FD) and have been used in clinical practice for<br />decades. The objective of this study is to evaluate whether QZWT granules can alleviate gastric hypersensitivity by improving<br />low-grade inflammation and the mucosal barrier in the duodenum in a functional dyspepsia-like (FD-like) model in rats.<br /><strong>Methods</strong>: FD-like gastric hypersensitivity in adulthood was induced by iodoacetamide (IA) in neonatal rats. Thirty-two<br />Sprague-Dawley (SD) rats were randomly classified into the control, model, QZWT, and Mosapride groups. Further, 0.2 mL<br />of 0.1% iodoacetamide and 2.0% sucrose administrated by oral gavage was used to establish the FD-like rats model (once<br />a day, for six consecutive days). Electromyography (EMG) was used to evaluate the visceral motor response to gastric<br />dilation. After treatment, the fluorescein isothiocyanate-glucan (FITC-glucan) test was performed to evaluate duodenal<br />permeability; enzyme-linked immunosorbent assay (ELISA) and quantitative polymerase chain reaction (qPCR) were used to<br />evaluate the pro-inflammatory cytokines for duodenal epithelial cells and the tight junction (TJ) proteins of the duodenal<br />mucosa. <strong>Results</strong>: The EMG score was elevated in the model group compared to the control group, but it decreased after<br />QZWT intervention; no changes were observed between the Mosapride and model groups. Compared with the control<br />group, rats in the gastric hypersensitivity model group showed a higher expression of interleukin-6 (IL-6) and tumor necrosis<br />factor-α (TNF-α) and lower expression of zona occludens protein 1 (ZO-1), occludin, and desmosome (DSG2) of junction<br />proteins (P < 0.05); while in the QZWT group, the expression of IL-6 and TNF-α was reduced (P < 0.01), and the expression<br />of ZO-1, occludin, and DSG2 was increased compared to the model group (P < 0.05). <strong>Conclusion</strong>: QZWT granules<br />alleviate visceral hypersensitivity in an FD-like model in rats by inhibiting inflammatory cytokines IL-6 and TNF-α and<br />regulating junction proteins.</p>yang yang, Xinyong Mao, Xiaoying Luo, Tao Zhang, Liang Wang, Ling Han, Ping Wang, Shuangshuang Fang, Jiande DZ Chen, Wei Wei
Copyright (c) 2024 Gut Microbiota and Integrative Wellness
https://creativecommons.org/licenses/by-nc-sa/4.0
https://www.hksmp.com/journals/gmiw/article/view/532Sun, 31 Mar 2024 00:00:00 +0800